Mass Cytometry:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 428 The next day, the cells were washed 1x in CyFACS, and 2x in Cell Acquisition Solution 429 (Standard BioTools), filtered through a 35 μm cell strainer (BD) before being acquired on a 430 CyTOF Helios (Standard BioTools). .. 431 432 Mass cytometry panels 433 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 434 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 435 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cd* anti-CD45RO Biolegend UCHL1 304202 50 110Cd** anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd** anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 175Lu anti-CXCR3 Biolegend G025H7 353733 100 176Yb anti-CD127 Standard Biotools A019D5 3176004B 100 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 *anti-CD45RO/106Cd was used for donor 1-4 (together with 198Pt as viability stain). ..
Article Title: Single cell suppression profiling of human regulatory T cells.
Article Snippet: The next day, the cells were washed 1× in CyFACS, and 2× in Cell Acquisition Solution (Standard BioTools, 201240), and filtered through a 35 μm cell strainer (BD, 352235) before being acquired on a CyTOFHelios (Standard BioTools). .. Nature Communications | (2025) 16:1325 11 Table 2 | Antibodies used for mass cytometry Label Target Manufacturer Clone Cat Number Titration Barcode Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface stain 106Cda anti-CD45RO Biolegend UCHL1 304202 50 110Cdb anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cdb anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Viability stain DCED palladium (106Cd/108Cd)a Dead cells Sigma - 574902 500 Cell-ID Cisplatin-198Pta Dead cells Standard BioTools - 201198 2000 Intracellular stain 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 148Nd anti-XBP1 Thermo Fisher Polyclonal PA5-27650 100 149Sm anti-Cytochrome C Biolegend 6H2.B4 612302 200 150Nd anti-Granzyme B Novus CLB-GB11 NBP1-50071 100 Nature Communications | (2025) 16:1325 12 .. FCS files were normalized and concatenated using CyTOF software (Standard BioTools, v. 7.0.8493) (Fig. S11a).
Titration:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 428 The next day, the cells were washed 1x in CyFACS, and 2x in Cell Acquisition Solution 429 (Standard BioTools), filtered through a 35 μm cell strainer (BD) before being acquired on a 430 CyTOF Helios (Standard BioTools). .. 431 432 Mass cytometry panels 433 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 434 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 435 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cd* anti-CD45RO Biolegend UCHL1 304202 50 110Cd** anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd** anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 175Lu anti-CXCR3 Biolegend G025H7 353733 100 176Yb anti-CD127 Standard Biotools A019D5 3176004B 100 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 *anti-CD45RO/106Cd was used for donor 1-4 (together with 198Pt as viability stain). ..
Article Title: Single cell suppression profiling of human regulatory T cells.
Article Snippet: The next day, the cells were washed 1× in CyFACS, and 2× in Cell Acquisition Solution (Standard BioTools, 201240), and filtered through a 35 μm cell strainer (BD, 352235) before being acquired on a CyTOFHelios (Standard BioTools). .. Nature Communications | (2025) 16:1325 11 Table 2 | Antibodies used for mass cytometry Label Target Manufacturer Clone Cat Number Titration Barcode Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface stain 106Cda anti-CD45RO Biolegend UCHL1 304202 50 110Cdb anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cdb anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Viability stain DCED palladium (106Cd/108Cd)a Dead cells Sigma - 574902 500 Cell-ID Cisplatin-198Pta Dead cells Standard BioTools - 201198 2000 Intracellular stain 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 148Nd anti-XBP1 Thermo Fisher Polyclonal PA5-27650 100 149Sm anti-Cytochrome C Biolegend 6H2.B4 612302 200 150Nd anti-Granzyme B Novus CLB-GB11 NBP1-50071 100 Nature Communications | (2025) 16:1325 12 .. FCS files were normalized and concatenated using CyTOF software (Standard BioTools, v. 7.0.8493) (Fig. S11a).
Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
Blocking Assay:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 428 The next day, the cells were washed 1x in CyFACS, and 2x in Cell Acquisition Solution 429 (Standard BioTools), filtered through a 35 μm cell strainer (BD) before being acquired on a 430 CyTOF Helios (Standard BioTools). .. 431 432 Mass cytometry panels 433 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 434 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 435 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cd* anti-CD45RO Biolegend UCHL1 304202 50 110Cd** anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd** anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 175Lu anti-CXCR3 Biolegend G025H7 353733 100 176Yb anti-CD127 Standard Biotools A019D5 3176004B 100 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 *anti-CD45RO/106Cd was used for donor 1-4 (together with 198Pt as viability stain). ..
Article Title: Single cell suppression profiling of human regulatory T cells.
Article Snippet: The next day, the cells were washed 1× in CyFACS, and 2× in Cell Acquisition Solution (Standard BioTools, 201240), and filtered through a 35 μm cell strainer (BD, 352235) before being acquired on a CyTOFHelios (Standard BioTools). .. Nature Communications | (2025) 16:1325 11 Table 2 | Antibodies used for mass cytometry Label Target Manufacturer Clone Cat Number Titration Barcode Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface stain 106Cda anti-CD45RO Biolegend UCHL1 304202 50 110Cdb anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cdb anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Viability stain DCED palladium (106Cd/108Cd)a Dead cells Sigma - 574902 500 Cell-ID Cisplatin-198Pta Dead cells Standard BioTools - 201198 2000 Intracellular stain 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 148Nd anti-XBP1 Thermo Fisher Polyclonal PA5-27650 100 149Sm anti-Cytochrome C Biolegend 6H2.B4 612302 200 150Nd anti-Granzyme B Novus CLB-GB11 NBP1-50071 100 Nature Communications | (2025) 16:1325 12 .. FCS files were normalized and concatenated using CyTOF software (Standard BioTools, v. 7.0.8493) (Fig. S11a).
Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
Staining:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 428 The next day, the cells were washed 1x in CyFACS, and 2x in Cell Acquisition Solution 429 (Standard BioTools), filtered through a 35 μm cell strainer (BD) before being acquired on a 430 CyTOF Helios (Standard BioTools). .. 431 432 Mass cytometry panels 433 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 434 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 435 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cd* anti-CD45RO Biolegend UCHL1 304202 50 110Cd** anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd** anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 175Lu anti-CXCR3 Biolegend G025H7 353733 100 176Yb anti-CD127 Standard Biotools A019D5 3176004B 100 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 *anti-CD45RO/106Cd was used for donor 1-4 (together with 198Pt as viability stain). ..
Article Title: Single cell suppression profiling of human regulatory T cells.
Article Snippet: The next day, the cells were washed 1× in CyFACS, and 2× in Cell Acquisition Solution (Standard BioTools, 201240), and filtered through a 35 μm cell strainer (BD, 352235) before being acquired on a CyTOFHelios (Standard BioTools). .. Nature Communications | (2025) 16:1325 11 Table 2 | Antibodies used for mass cytometry Label Target Manufacturer Clone Cat Number Titration Barcode Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface stain 106Cda anti-CD45RO Biolegend UCHL1 304202 50 110Cdb anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cdb anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Viability stain DCED palladium (106Cd/108Cd)a Dead cells Sigma - 574902 500 Cell-ID Cisplatin-198Pta Dead cells Standard BioTools - 201198 2000 Intracellular stain 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 148Nd anti-XBP1 Thermo Fisher Polyclonal PA5-27650 100 149Sm anti-Cytochrome C Biolegend 6H2.B4 612302 200 150Nd anti-Granzyme B Novus CLB-GB11 NBP1-50071 100 Nature Communications | (2025) 16:1325 12 .. FCS files were normalized and concatenated using CyTOF software (Standard BioTools, v. 7.0.8493) (Fig. S11a).
Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
Recombinase Polymerase Amplification:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 428 The next day, the cells were washed 1x in CyFACS, and 2x in Cell Acquisition Solution 429 (Standard BioTools), filtered through a 35 μm cell strainer (BD) before being acquired on a 430 CyTOF Helios (Standard BioTools). .. 431 432 Mass cytometry panels 433 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 434 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 435 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cd* anti-CD45RO Biolegend UCHL1 304202 50 110Cd** anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd** anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 175Lu anti-CXCR3 Biolegend G025H7 353733 100 176Yb anti-CD127 Standard Biotools A019D5 3176004B 100 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 *anti-CD45RO/106Cd was used for donor 1-4 (together with 198Pt as viability stain). ..
Article Title: Single cell suppression profiling of human regulatory T cells.
Article Snippet: The next day, the cells were washed 1× in CyFACS, and 2× in Cell Acquisition Solution (Standard BioTools, 201240), and filtered through a 35 μm cell strainer (BD, 352235) before being acquired on a CyTOFHelios (Standard BioTools). .. Nature Communications | (2025) 16:1325 11 Table 2 | Antibodies used for mass cytometry Label Target Manufacturer Clone Cat Number Titration Barcode Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 μm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface stain 106Cda anti-CD45RO Biolegend UCHL1 304202 50 110Cdb anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cdb anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Viability stain DCED palladium (106Cd/108Cd)a Dead cells Sigma - 574902 500 Cell-ID Cisplatin-198Pta Dead cells Standard BioTools - 201198 2000 Intracellular stain 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 148Nd anti-XBP1 Thermo Fisher Polyclonal PA5-27650 100 149Sm anti-Cytochrome C Biolegend 6H2.B4 612302 200 150Nd anti-Granzyme B Novus CLB-GB11 NBP1-50071 100 Nature Communications | (2025) 16:1325 12 .. FCS files were normalized and concatenated using CyTOF software (Standard BioTools, v. 7.0.8493) (Fig. S11a).
Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
Biomarker Discovery:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
Flow Cytometry:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
Marker:Article Title: Single cell suppression profiling of human regulatory T cells
Article Snippet: 3 nature portfolio | reporting sum m ary April2023 Antibodies Antibodies used FACS sorting Label Target Manufacturer Clone Cat Number Titration BV711 CD45RA Biolegend HI100 304138 20 APC-Cy7 CD4 BD RPA-T4 557871 20 AF647 CD127 Biolegend A019D5 351318 20 BV421 CXCR5 BD RF8B2 562747 20 PerCP-Cy5.5 CD45RO BD UCHL1 560607 20 .. V500 CD19 (to exclude potential B cell doublets) BD HIB19 561121 20 Barcode Label Target Manufacturer Clone Cat Number Titration Fc block Biolegend - 422302 50 89Y anti-CD45 Standard Biotools HI30 3089003B 50 113In anti-CD45 Biolegend HI30 304002 100 115In anti-CD45 Biolegend HI30 304002 100 194Pt anti-CD45 Biolegend HI30 304002 100 195Pt anti-CD45 Biolegend HI30 304002 100 196Pt anti-CD45 Biolegend HI30 304002 100 198Pt anti-CD45 Biolegend HI30 304002 100 Pre-stain Label Target Manufacturer Clone Cat Number Titration 153Eu anti-CD45RA Biolegend HI100 304102 100 154Sm anti-CXCR3 Biolegend G025H7 353733 100 155Gd anti-CCR7 Biolegend G043H7 353202 100 160gd anti-CCR4 Biolegend L291H4 359402 100 163Dy anti-CCR6 Biolegend G034E3 353427 50 127I IdU (125 µm) Standard BioTools - 201192A 600 NA anti-CXCR5 BD RF8B2 552118 25 NA Puromycin (1 mg/ml) Sigma - P8833 300 NA BrU (5mM) Sigma - 850187 20 Surface Stain Label Target Manufacturer Clone Cat Number Titration 106Cdanti-CD45RO Biolegend UCHL1 304202 50 110Cd anti-CD3 Thermo Fisher Scientific MEM-57 MA544054 100 110Cd anti-CD21 Biolegend Bu32 354902 100 111Cd anti-CD4 Biolegend RPA-T4 300502 100 112Cd anti-CD8 Biolegend RPA-T8 301002 50 114Cd anti-CD39 Biolegend A1 328221 100 116Cd anti-CD19 Biolegend HIB19 302202 100 147Sm anti-CD86 Biolegend IT2.2 305402 50 151Eu anti-IgD Biolegend IA6-2 348202 100 156Gd anti-CD274/PD-L1 Standard BioTools 29E.2A3 3156026B 100 158Gd anti-CD27 Standard BioTools L128 3158010B 200 159Tb anti-TIM3 Standard BioTools F38-2E2 3159037B 100 165Ho anti-Biotin (for CXCR5) Standard BioTools 1D4C5 3165012B 50 166Er anti-PD1 Biolegend EH12.2H7 329941 100 169Tm anti-CD25 Standard BioTools 2A3 3169003B 200 171Yb anti-CD98 BD UM7F8 556074 200 172Yb anti-CD38 Standard BioTools HIT2 3172007B 100 173Yb anti-CD11c Biolegend S-HCL-3 371502 100 174Yb anti-HLA-DR Biolegend L243 307602 400 209Bi anti-TIGIT Standard Biotools MBSA43 3209013B 100 Intracellular Stain Label Target Manufacturer Clone Cat Number Titration 140Ce anti-H3K27me3 Active motive 323 61017 200 141Pr anti-Puromycin Merck 12D10 MABE343 200 142Nd anti-Cleaved caspase 3 Standard BioTools D3E9 3142004A 100 143Nd anti-TCF1 Biolegend 7F11A10 655202 50 144Nd anti-FITC (for CFSE) Standard BioTools FIT-22 3144006B 100 145Nd anti-Helios Biolegend 22F6 137202 100 146Nd Anti-OGDH Thermo Fisher Scientific Polyclonal PA528195 100 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants Flow Cytometry Plots Confirm that: The axis labels state the marker and fluorochrome used (e.g. CD4-FITC). ..
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